Female Germline Stem Cell Culture Training Course
Technical Training
As a the world’s leading Lab for Female germline stem cell (FGSC) study[1-3], our mission is to help and support researchers for their FGSC study in the world.
FGSC training courses provide hands-on training for attendants to learn ovarian dissection, dissociation, and the two-step enzymatic digestion as well as FGSC purification, culture, and transplantation back into mouse ovary. Researchers will give personal instruction and information of FGSC biology, and protocols on the care, maintenance of FGSC, with continued guidance and feedback during and following the training course.
Upon completion of the course, you will have a thorough understanding of FGSC biology and current practices in the field. In addition, you will learn to overcome challenges involved in key stem cell assay procedures and become confident in performing specialized practical germline stem cell techniques in your own laboratory setting. Researchers in field of reproductive biology, or reproductive medicine, or stem cell biology are strongly encouraged.
The Training Course Covers
1. Prepare proper culture medium for FGSCs
2. Culture and preparing of feeder layer
3. Aseptic dissection of mouse ovary
4. FGSC isolation by two-step enzymatic digestion
5. Magnetic purification of FGSCs
6. Culture and proper passaging (splitting) of FGSCs
7. Cryopreservation of FGSCs
8. Transplantation of mouse FGSCs for in vivo assay
9. Post-laboratory Q&A and troubleshooting of techniques
Place for training course
Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China
Duration of training course
Two to five days.
Cost
Please contact us at jiwu@sjtu.edu.cn for a current fee schedule. Attendees are responsible for their own travel, but we will provide accommodations and meals.
Contact Information
Prof. Ji Wu
Phone: (86) 21-34207263; (86) 21-13524892168
Email: jiwu@sjtu.edu.cn
Reference:
1. Zou K, Yuan Z, Yang Z, Luo H, Sun K, Zhou L, Xiang J, Shi L, Yu Q, Zhang Y, Hou R, Wu J.* Production of offspring from a germline stem cell line derived from ovaries. Nature Cell Biology 2009; 11: 631-636.
2. Zhang Y., Yang Z., Yang Y, Wang S., Shi L., Xie W., Sun K., Zou K., Wang L., Xiong J., Xiang J, Wu J.* Production of transgenic mice by random recombination of targeted genes in female germline stem cells. Journal of Molecular Cell Biology 2011; 3: 132-141
3. Zou K., Hou L., Sun K., Xie W., Wu J.* Improved Efficiency of Female Germline Stem Cell Purification Using Fragilis-Based Magnetic Bead Sorting. Stem Cells and Development 2011; 20: 2197-2204.
